• Western blot analysis of extracts of various cell lines, using ERAP2 antibody (STJ111527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of various lysates using ERAP2 Rabbit polyclonal antibody (STJ111527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ERAP2 antibody (1-170) (STJ111527)

SKU:
STJ111527

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ERAP2 (1-170) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERAP2
Gene ID: 64167
Uniprot ID: ERAP2_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ERAP2 (NP_071745.1).
Immunogen Sequence: MFHSSAMVNSHRKPMFNIHR GFYCLTAILPQICICSQFSV PSSYHFTEDPGAFPVATNGE RFPWQELRLPSVVIPLHYDL FVHPNLTSLDFVASEKIEVL VSNATQFIILHSKDLEITNA TLQSEEDSRYMKPGKELKVL SYPAHEQIALLVPEKLTPHL KYYVAMDFQA
Post Translational Modifications N-glycosylated.
Function Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.
Protein Name Endoplasmic Reticulum Aminopeptidase 2
Leukocyte-Derived Arginine Aminopeptidase
L-Rap
Database Links Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Endoplasmic Reticulum Aminopeptidase 2 antibody
Anti-Leukocyte-Derived Arginine Aminopeptidase antibody
Anti-L-Rap antibody
Anti-ERAP2 antibody
Anti-LRAP antibody

Information sourced from Uniprot.org

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