• Western blot analysis of extracts of various cell lines, using EPO antibody (STJ114309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EPO antibody (STJ114309)

SKU:
STJ114309

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPO is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPO
Gene ID: 2056
Uniprot ID: EPO_HUMAN
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human EPO (NP_000790.2).
Immunogen Sequence: AVLRGQALLVNSSQPWEPLQ LHVDKAVSGLRSLTTLLRAL GAQKEAISPPDAASAAPLRT ITADTFRKLFRVYSNFLRGK LKLYTGEACRTGDR
Tissue Specificity Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Function Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
Protein Name Erythropoietin
Epoetin
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-9006335
Reactome: R-HSA-9027276
Reactome: R-HSA-9027277
Reactome: R-HSA-9027283
Reactome: R-HSA-9027284
Cellular Localisation Secreted
Alternative Antibody Names Anti-Erythropoietin antibody
Anti-Epoetin antibody
Anti-EPO antibody

Information sourced from Uniprot.org

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