• Western blot analysis of various lysates using EIF4G3 Rabbit polyclonal antibody (STJ116828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF4G3 antibody (1000-1200) (STJ116828)

SKU:
STJ116828

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF4G3 (1000-1200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4G3
Gene ID: 8672
Uniprot ID: IF4G3_HUMAN
Immunogen Region: 1000-1200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_003751.2).
Immunogen Sequence: HKEAKIEEQEEQRKVQQLMT KEKRRPGVQRVDEGGWNTVQ GAKNSRVLDPSKFLKITKPT IDEKIQLVPKAQLGSWGKGS SGGAKASETDALRSSASSLN RFSALQPPAPSGSTPSTPVE FDSRRTLTSRGSMGREKNDK PLPSATARPNTFMRGGSSKD LLDNQSQEEQRREMLETVKQ LTGGVDVERNSTEAERNKTR E
Post Translational Modifications Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
Function Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Functional homolog of EIF4G1.
Protein Name Eukaryotic Translation Initiation Factor 4 Gamma 3
Eif-4-Gamma 3
Eif-4g 3
Eif4g 3
Eif-4-Gamma Ii
Eif4gii
Database Links Reactome: R-HSA-1169408
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4 Gamma 3 antibody
Anti-Eif-4-Gamma 3 antibody
Anti-Eif-4g 3 antibody
Anti-Eif4g 3 antibody
Anti-Eif-4-Gamma Ii antibody
Anti-Eif4gii antibody
Anti-EIF4G3 antibody

Information sourced from Uniprot.org

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