• Immunohistochemistry analysis of paraffin-embedded human breast cancer using EIF4G1 Rabbit polyclonal antibody (STJ116294) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using EIF4G1 antibody (STJ116294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60S.

Anti-EIF4G1 antibody (400-500) (STJ116294)

SKU:
STJ116294

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF4G (400-500) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4G1
Gene ID: 1981
Uniprot ID: IF4G1_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
Immunogen Sequence: PEELLNGAPSPPAVDLSPVS EPEEQAKEVTASMAPPTIPS ATPATAPSATSPAQEEEMEE EEEEEEGEAGEAGEAESEKG GEELLPPESTPIPANLSQNL E
Post Translational Modifications Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1. Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
Function Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation.
Protein Name Eukaryotic Translation Initiation Factor 4 Gamma 1
Eif-4-Gamma 1
Eif-4g 1
Eif-4g1
P220
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-156827
Reactome: R-HSA-166208
Reactome: R-HSA-429947
Reactome: R-HSA-450408
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-9010553
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Stress Granule
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4 Gamma 1 antibody
Anti-Eif-4-Gamma 1 antibody
Anti-Eif-4g 1 antibody
Anti-Eif-4g1 antibody
Anti-P220 antibody
Anti-EIF4G1 antibody
Anti-EIF4F antibody
Anti-EIF4G antibody
Anti-EIF4GI antibody

Information sourced from Uniprot.org

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