• Western blot analysis of extracts from normal (control) and ECH1 knockout (KO) 293T cells, using ECH1 antibody (STJ11100744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ECH1 antibody (1-328) (STJ11100744)

SKU:
STJ11100744

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECH1 (1-328) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECH1
Gene ID: 1891
Uniprot ID: ECH1_HUMAN
Immunogen Region: 1-328
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
Immunogen Sequence: MAAGIVASRRLRDLLTRRLT GSNYPGLSISLRLTGSSAQE EASGVALGEAPDHSYESLRV TSAQKHVLHVQLNRPNKRNA MNKVFWREMVECFNKISRDA DCRAVVISGAGKMFTAGIDL MDMASDILQPKGDDVARISW YLRDIITRYQETFNVIERCP KPVIAAVHGGCIGGGVDLVT ACDIRYCAQDAFFQVKEVDV GLAADVGTLQRLPKVIGNQS LVNELAFTARKMMADEALG
Function Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
Protein Name Delta(3 -5-Delta(2 -4-Dienoyl-Coa Isomerase - Mitochondrial
Database Links Reactome: R-HSA-9033241
Cellular Localisation Mitochondrion
Peroxisome
Alternative Antibody Names Anti-Delta(3 -5-Delta(2 -4-Dienoyl-Coa Isomerase - Mitochondrial antibody
Anti-ECH1 antibody

Information sourced from Uniprot.org

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