| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DYNLL1 |
| Gene ID: | 8655 |
| Uniprot ID: | DYL1_HUMAN |
| Immunogen Region: | 1-89 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-89 of human DYNLL1 (NP_003737.1). |
| Immunogen Sequence: | MCDRKAVIKNADMSEEMQQD SVECATQALEKYNIEKDIAA HIKKEFDKKYNPTWHCIVGR NFGSYVTHETKHFIYFYLGQ VAILLFKSG |
| Tissue Specificity | Ubiquitous. Expressed in testis. |
| Post Translational Modifications | Phosphorylation at Ser-88 promotes recruitment to DNA double-strand breaks (DSBs) by TP53BP1 and ability to inhibit MRE11. Phosphorylation at Ser-88 appears to control the dimer-monomer transition. it is phosphorylated at Ser-88 by PAK1, however, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro. |
| Function | Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. In addition to its role in cytoskeleton and transport, acts as a protein-protein adapter, which inhibits and/or sequesters target proteins. Involved in the response to DNA damage by acting as a key regulator of DNA end resection: when phosphorylated at Ser-88, recruited to DNA double-strand breaks (DSBs) by TP53BP1 and acts by disrupting MRE11 dimerization, thereby inhibiting DNA end resection. In a subset of DSBs, DYNLL1 remains unphosphorylated and promotes the recruitment of the Shieldin complex. Binds and inhibits the catalytic activity of neuronal nitric oxide synthase/NOS1. Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1. Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity. |
| Protein Name | Dynein Light Chain 1 - Cytoplasmic 8 Kda Dynein Light Chain Dlc8 Dynein Light Chain Lc8-Type 1 Protein Inhibitor Of Neuronal Nitric Oxide Synthase Pin |
| Database Links | Reactome: R-HSA-111446 Reactome: R-HSA-141444 Reactome: R-HSA-1632852 Reactome: R-HSA-2132295 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-2565942 Reactome: R-HSA-3371497 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-5620912 Reactome: R-HSA-5620924 Reactome: R-HSA-5663220 Reactome: R-HSA-6798695 Reactome: R-HSA-6807878 Reactome: R-HSA-6811436 Reactome: R-HSA-68877 Reactome: R-HSA-8854518 Reactome: R-HSA-9609690 Reactome: R-HSA-9646399 Reactome: R-HSA-9648025 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Chromosome Nucleus Mitochondrion Upon Induction Of Apoptosis Translocates Together With Bcl2l11 To Mitochondria Recruited To Dna Double-Strand Breaks (Dsbs) By Tp53bp1 When Phosphorylated At Ser-88 |
| Alternative Antibody Names | Anti-Dynein Light Chain 1 - Cytoplasmic antibody Anti-8 Kda Dynein Light Chain antibody Anti-Dlc8 antibody Anti-Dynein Light Chain Lc8-Type 1 antibody Anti-Protein Inhibitor Of Neuronal Nitric Oxide Synthase antibody Anti-Pin antibody Anti-DYNLL1 antibody Anti-DLC1 antibody Anti-DNCL1 antibody Anti-DNCLC1 antibody Anti-HDLC1 antibody |
Information sourced from Uniprot.org