• Western blot analysis of various lysates using BANF1 Rabbit polyclonal antibody (STJ116766) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BANF1 antibody (1-89) (STJ116766)

SKU:
STJ116766

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BANF1 (1-89) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BANF1
Gene ID: 8815
Uniprot ID: BAF_HUMAN
Immunogen Region: 1-89
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (NP_001137457.1).
Immunogen Sequence: MTTSQKHRDFVAEPMGEKPV GSLAGIGEVLGKKLEERGFD KAYVVLGQFLVLKKDEDLFR EWLKDTCGANAKQSRDCFGC LREWCDAFL
Tissue Specificity Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
Post Translational Modifications Ser-4 is the major site of phosphorylation as compared to Thr-2 and Thr-3. Phosphorylation on Thr-2.Thr-3 and Ser-4 disrupts its ability to bind DNA and reduces its ability to bind LEM domain-containing proteins. Non phosphorylated BAF seems to enhance binding between EMD and LMNA. Dephosphorylated by protein phosphatase 2A (PP2A) following interaction with ANKLE2/LEM4 during mitotic exit, leading to mitotic nuclear envelope reassembly. (Microbial infection) Phosphorylated by poxvirus B1 kinase (VPK1) on serine and threonine residues, leading to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity. (Microbial infection) Phosphorylated at the N-terminus by vaccinia virus (VacV) B1 kinase, leading to BANF1 relocalization to the cytoplasm, loss of dimerization and impaired DNA binding activity. Hyperphosphorylation is linked to the loss of ability to suppress vaccinia virus replication.
Function Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA. Contains two non-specific double-stranded DNA (dsDNA)-binding sites which promote DNA cross-bridging. Plays a key role in nuclear membrane reformation at the end of mitosis by driving formation of a single nucleus in a spindle-independent manner. Transiently cross-bridges anaphase chromosomes via its ability to bridge distant DNA sites, leading to the formation of a dense chromatin network at the chromosome ensemble surface that limits membranes to the surface. Also acts as a negative regulator of innate immune activation by restricting CGAS activity toward self-DNA upon acute loss of nuclear membrane integrity. Outcompetes CGAS for DNA-binding, thereby preventing CGAS activation and subsequent damaging autoinflammatory responses. Also involved in DNA damage response: interacts with PARP1 in response to oxidative stress, thereby inhibiting the ADP-ribosyltransferase activity of PARP1. Involved in the recognition of exogenous dsDNA in the cytosol: associates with exogenous dsDNA immediately after its appearance in the cytosol at endosome breakdown and is required to avoid autophagy. In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication. (Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
Protein Name Barrier-To-Autointegration Factor
Breakpoint Cluster Region Protein 1 Cleaved Into - Barrier-To-Autointegration Factor - N-Terminally Processed
Database Links Reactome: R-HSA-162592
Reactome: R-HSA-164843
Reactome: R-HSA-175567
Reactome: R-HSA-177539
Reactome: R-HSA-180689
Reactome: R-HSA-180910
Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Cellular Localisation Nucleus
Chromosome
Nucleus Envelope
Cytoplasm
Significantly Enriched At The Nuclear Inner Membrane
Diffusely Throughout The Nucleus During Interphase And Concentrated At The Chromosomes During The M-Phase
The Phosphorylated Form (By Vrk1) Shows A Cytoplasmic Localization Whereas The Unphosphorylated Form Locates Almost Exclusively In The Nucleus
May Be Included In Hiv-1 Virions Via Its Interaction With Viral Gag Polyprotein
Alternative Antibody Names Anti-Barrier-To-Autointegration Factor antibody
Anti-Breakpoint Cluster Region Protein 1 Cleaved Into - Barrier-To-Autointegration Factor - N-Terminally Processed antibody
Anti-BANF1 antibody
Anti-BAF antibody
Anti-BCRG1 antibody

Information sourced from Uniprot.org

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