• Immunohistochemistry analysis of paraffin-embedded human colon using DUSP6 Rabbit monoclonal antibody (STJ11101230) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DUSP6 Rabbit monoclonal antibody (STJ11101230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-DUSP6 antibody (282-381) [S0MR] (STJ11101230)

SKU:
STJ11101230

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DUSP6 (282-381) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUSP6
Gene ID: 1848
Uniprot ID: DUS6_HUMAN
Immunogen Region: 282-381
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 282-381 of human DUSP6 (Q16828).
Immunogen Sequence: ARGKNCGVLVHCLAGISRSV TVTVAYLMQKLNLSMNDAYD IVKMKKSNISPNFNFMGQLL DFERTLGLSSPCDNRVPAQQ LYFTTPSNQNVYQVDSLQST
Tissue Specificity Expressed in keratinocytes (at protein level).
Function Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain. Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.
Protein Name Dual Specificity Protein Phosphatase 6
Dual Specificity Protein Phosphatase Pyst1
Mitogen-Activated Protein Kinase Phosphatase 3
Map Kinase Phosphatase 3
Mkp-3
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-202670
Reactome: R-HSA-5675221
Reactome: R-HSA-9652817
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Dual Specificity Protein Phosphatase 6 antibody
Anti-Dual Specificity Protein Phosphatase Pyst1 antibody
Anti-Mitogen-Activated Protein Kinase Phosphatase 3 antibody
Anti-Map Kinase Phosphatase 3 antibody
Anti-Mkp-3 antibody
Anti-DUSP6 antibody
Anti-MKP3 antibody
Anti-PYST1 antibody

Information sourced from Uniprot.org

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