• Western blot analysis of extracts of U-87MG cells, using DRD5 antibody (STJ23434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of mouse brain, using DRD5 antibody (STJ23434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DRD5 antibody (323-477) (STJ23434)

SKU:
STJ23434

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DRD5 (323-477) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DRD5
Gene ID: 1816
Uniprot ID: DRD5_HUMAN
Immunogen Region: 323-477
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 323-477 of human DRD5 (NP_000789.1).
Immunogen Sequence: SGHPEGPPAGFPCVSETTFD VFVWFGWANSSLNPVIYAFN ADFQKVFAQLLGCSHFCSRT PVETVNISNELISYNQDIVF HKEIAAAYIHMMPNAVTPGN REVDNDEEEGPFDRMFQIYQ TSPDGDPVAESVWELDCEGE ISLDKITPFTPNGFH
Tissue Specificity Neuron-specific, localized primarily within limbic regions of the brain.
Function Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Protein Name D(1b Dopamine Receptor
D(5 Dopamine Receptor
D1beta Dopamine Receptor
Dopamine D5 Receptor
Database Links Reactome: R-HSA-390651
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-D(1b Dopamine Receptor antibody
Anti-D(5 Dopamine Receptor antibody
Anti-D1beta Dopamine Receptor antibody
Anti-Dopamine D5 Receptor antibody
Anti-DRD5 antibody
Anti-DRD1B antibody
Anti-DRD1L2 antibody

Information sourced from Uniprot.org

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