| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DLC1 |
| Gene ID: | 10395 |
| Uniprot ID: | RHG07_HUMAN |
| Immunogen Region: | 204-463 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human DLC1 (NP_079043.3). |
| Immunogen Sequence: | DAPKVNAVDTLNVKDIAPEK QLLNSAVIAQQRRKPDPPKD ENERSTCNVVQNEFLDTPCT NRGLPLLKTDFGSCLLQPPS CPNGMSAENGLEKSGFSQHQ NKSPPKVKAEDGMQCLQLKE TLATQEPTDNQVRLRKRKEI REDRDRARLDSMVLLIMKLD QLDQDIENALSTSSSPSGTP TNLRRHVPDLESGSESGADT ISVNQTRVNLSSDTESTDLP SSTPVANSGTKPKTTAIQG |
| Tissue Specificity | Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus. |
| Function | Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality. Required for growth factor-induced epithelial cell migration.in resting cells, interacts with TNS3 while PTEN interacts with the p85 regulatory subunit of the PI3K kinase complex but growth factor stimulation induces phosphorylation of TNS3 and PTEN, causing them to change their binding preference so that PTEN interacts with DLC1 and TNS3 interacts with p85. The PTEN-DLC1 complex translocates to the posterior of migrating cells to activate RHOA while the TNS3-p85 complex translocates to the leading edge of migrating cells to promote RAC1 activation. |
| Protein Name | Rho Gtpase-Activating Protein 7 Deleted In Liver Cancer 1 Protein Dlc-1 Hp Protein Rho-Type Gtpase-Activating Protein 7 Start Domain-Containing Protein 12 Stard12 Star-Related Lipid Transfer Protein 12 |
| Database Links | Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013406 |
| Cellular Localisation | Cytoplasm Cell Junction Focal Adhesion Membrane Peripheral Membrane Protein Colocalizes With Ef1a1 At Actin-Rich Regions In The Cell Periphery |
| Alternative Antibody Names | Anti-Rho Gtpase-Activating Protein 7 antibody Anti-Deleted In Liver Cancer 1 Protein antibody Anti-Dlc-1 antibody Anti-Hp Protein antibody Anti-Rho-Type Gtpase-Activating Protein 7 antibody Anti-Start Domain-Containing Protein 12 antibody Anti-Stard12 antibody Anti-Star-Related Lipid Transfer Protein 12 antibody Anti-DLC1 antibody Anti-ARHGAP7 antibody Anti-KIAA1723 antibody Anti-STARD12 antibody |
Information sourced from Uniprot.org