| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DIDO1 (1-300) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DIDO1 |
| Gene ID: |
11083 |
| Uniprot ID: |
DIDO1_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DIDO1 (NP_071388.2). |
| Immunogen Sequence: |
MDDKGDPSNEEAPKAIKPTS KEFRKTWGFRRTTIAKREGA GDAEADPLEPPPPQQQLGLS LRRSGRQPKRTERVEQFLTI ARRRGRRSMPVSLEDSGEPT SCPATDAETASEGSVESASE TRSGPQSASTAVKERPASSE KVKGGDDHDDTSDSDSDGLT LKELQNRLRRKREQEPTERP LKGIQSRLRKKRREEGPAET VGSEASDTVEGVLPSKQEPE NDQGVVSQAGKDDRESKLE |
| Tissue Specificity | Ubiquitous. |
| Function | Putative transcription factor, weakly pro-apoptotic when overexpressed. Tumor suppressor. Required for early embryonic stem cell development. Isoform 2: Displaces isoform 4 at the onset of differentiation, required for repression of stemness genes. |
| Protein Name | Death-Inducer Obliterator 1Dio-1Hdido1Death-Associated Transcription Factor 1Datf-1 |
| Database Links | Reactome: R-HSA-1221632 Q9BTC0-1 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonSpindleTranslocates To The Nucleus After Pro-Apoptotic StimuliTranslocates To The Mitotic Spindle Upon Loss Of Interaction With H3k4me3 During Early Mitosis |
| Alternative Antibody Names | Anti-Death-Inducer Obliterator 1 antibodyAnti-Dio-1 antibodyAnti-Hdido1 antibodyAnti-Death-Associated Transcription Factor 1 antibodyAnti-Datf-1 antibodyAnti-DIDO1 antibodyAnti-C20orf158 antibodyAnti-DATF1 antibodyAnti-KIAA0333 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
