• Western blot analysis of extracts of various cell lines, using DIAPH1 antibody (STJ28339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using DIAPH1 antibody (STJ28339). Blue: DAPI for nuclear staining.

Anti-DIAPH1 antibody (1149-1263) (STJ28339)

SKU:
STJ28339

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DIAPH1 (1149-1263) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DIAPH1
Gene ID: 1729
Uniprot ID: DIAP1_HUMAN
Immunogen Region: 1149-1263
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1149-1263 of human DIAPH1 (NP_001073280.1).
Immunogen Sequence: TEEKMRRAKLAKEKAEKERL EKQQKREQLIDMNAEGDETG VMDSLLEALQSGAAFRRKRG PRQANRKAGCAVTSLLASEL TKDDAMAAVPAKVSKNSETF PTILEEAKELVGRAS
Tissue Specificity Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets.
Post Translational Modifications Phosphorylation at Thr-768 is stimulated by cAMP and regulates stability, complex formation and mitochondrial movement.
Function Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane. In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Plays a role in brain development. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
Protein Name Protein Diaphanous Homolog 1
Diaphanous-Related Formin-1
Drf1
Database Links Reactome: R-HSA-5663220
Reactome: R-HSA-6785631
Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013405
Reactome: R-HSA-9035034
Cellular Localisation Cell Membrane
Cell Projection
Ruffle Membrane
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Nucleus
Membrane Ruffles
Especially At The Tip Of Ruffles
Of Motile Cells
Alternative Antibody Names Anti-Protein Diaphanous Homolog 1 antibody
Anti-Diaphanous-Related Formin-1 antibody
Anti-Drf1 antibody
Anti-DIAPH1 antibody
Anti-DIAP1 antibody

Information sourced from Uniprot.org

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