| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DGKE (40-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DGKE |
| Gene ID: |
8526 |
| Uniprot ID: |
DGKE_HUMAN |
| Immunogen Region: |
40-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1). |
| Immunogen Sequence: |
CSLQRSRRQLHRRDIFRKSK HGWRDTDLFSQPTYCCVCAQ HILQGAFCDCCGLRVDEGCL RKADKRFQCKEIMLKNDTKV LDAMPHHWIRGNVPLCSYCM VCKQQCGCQPKLCDYRCIWC QKTVHDECMKNSLKNEKCDF GEFKNLIIPPSYLTSINQMR KDKKTDYEVLASKLGKQWTP LIILANSRSGTNMGEGLLGE FRILLNPVQVFDVTKTPPIK A |
| Tissue Specificity | Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level). |
| Function | Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently. |
| Protein Name | Diacylglycerol Kinase EpsilonDag Kinase EpsilonDiglyceride Kinase EpsilonDgk-Epsilon |
| Database Links | Reactome: R-HSA-114508 |
| Cellular Localisation | MembraneSingle-Pass Membrane ProteinCytoplasm |
| Alternative Antibody Names | Anti-Diacylglycerol Kinase Epsilon antibodyAnti-Dag Kinase Epsilon antibodyAnti-Diglyceride Kinase Epsilon antibodyAnti-Dgk-Epsilon antibodyAnti-DGKE antibodyAnti-DAGK5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
