• Western blot analysis of extracts of C6 cells, using DDIT3/CHOP antibody (STJ113090) at 1:1000 dilution. C6 cells were treated by tunicamycin (2 Mu g/ml) for 8 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using DDIT3/CHOP rabbit polyclonal antibody (STJ113090) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using DDIT3/CHOP rabbit polyclonal antibody (STJ113090) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300 Mu g extracts of C2C12 tunicamycin cells using 3 Mu g DDIT3/CHOP antibody (STJ113090). Western blot was performed from the immunoprecipitate using DDIT3/CHOP antibody (STJ113090) at a dilution of 1:500.

Anti-DDIT3 antibody (39-169) (STJ113090)

SKU:
STJ113090

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDIT3/CHOP (39-169) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDIT3
Gene ID: 1649
Uniprot ID: DDIT3_HUMAN
Immunogen Region: 39-169
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 39-169 of human DDIT3/CHOP (NP_004074.2).
Immunogen Sequence: VSPPGNEEEESKIFTTLDPA SLAWLT
Post Translational Modifications Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.
Function Multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates.expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress. Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling.
Protein Name Dna Damage-Inducible Transcript 3 Protein
Ddit-3
C/Ebp Zeta
C/Ebp-Homologous Protein
Chop
C/Ebp-Homologous Protein 10
Chop-10
Ccaat/Enhancer-Binding Protein Homologous Protein
Growth Arrest And Dna Damage-Inducible Protein Gadd153
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-381183
Reactome: R-HSA-9614657
Reactome: R-HSA-9633012
Reactome: R-HSA-9648895
Cellular Localisation Cytoplasm
Nucleus
Present In The Cytoplasm Under Non-Stressed Conditions And Er Stress Leads To Its Nuclear Accumulation
Alternative Antibody Names Anti-Dna Damage-Inducible Transcript 3 Protein antibody
Anti-Ddit-3 antibody
Anti-C/Ebp Zeta antibody
Anti-C/Ebp-Homologous Protein antibody
Anti-Chop antibody
Anti-C/Ebp-Homologous Protein 10 antibody
Anti-Chop-10 antibody
Anti-Ccaat/Enhancer-Binding Protein Homologous Protein antibody
Anti-Growth Arrest And Dna Damage-Inducible Protein Gadd153 antibody
Anti-DDIT3 antibody
Anti-CHOP antibody
Anti-CHOP10 antibody
Anti-GADD153 antibody

Information sourced from Uniprot.org

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