• Western blot analysis of extracts of various cell lines, using Doublecortin antibody (STJ116821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of Neuro-2a cells using Doublecortin rabbit polyclonal antibody (STJ116821) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Anti-DCX antibody (265-365) (STJ116821)

Anti-DCX antibody (265-365) (STJ116821)

SKU:
STJ116821

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCX (265-365) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCX
Gene ID: 1641
Uniprot ID: DCX_HUMAN
Immunogen Region: 265-365
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 265-365 of human Doublecortin (NP_835365.1).
Immunogen Sequence: SLDENECRVMKGNPSATAGP KASPTPQKTSAKSPGPMRRS KSPADSGNDQDANGTSSSQL STPKSKQSPISTPTSPGSLR KHKDLYLPLSLDDSDSLGDS M
Tissue Specificity Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
Post Translational Modifications Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Protein Name Neuronal Migration Protein Doublecortin
Doublin
Lissencephalin-X
Lis-X
Database Links Reactome: R-HSA-447043
Cellular Localisation Cytoplasm
Cell Projection
Neuron Projection
Localizes At Neurite Tips
Alternative Antibody Names Anti-Neuronal Migration Protein Doublecortin antibody
Anti-Doublin antibody
Anti-Lissencephalin-X antibody
Anti-Lis-X antibody
Anti-DCX antibody
Anti-DBCN antibody
Anti-LISX antibody

Information sourced from Uniprot.org

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