• Immunoprecipitation of DBI in 300 µg extracts from HeLa cells using 3 µg [KO Validated] DBI Rabbit polyclonal antibody (STJ119997). Western blot analysis was performed using [KO Validated] DBI Rabbit polyclonal antibody (STJ119997) at 1:1000 dilution.
  • Western blot analysis of lysates from wild type (WT) and DBI knockout (KO) 293T cells, using [KO Validated] DBI Rabbit polyclonal antibody (STJ119997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DBI antibody (1-114) (STJ119997)

SKU:
STJ119997

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DBI (1-114) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DBI
Gene ID: 1622
Uniprot ID: ACBP_HUMAN
Immunogen Region: 1-114
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human DBI (NP_001171513.1).
Immunogen Sequence: MWGDLWLLPPASANPGTGTE AEFEKAAEEVRHLKTKPSDE EMLFIYGHYKQATVGDINTE RPGMLDFTGKAKWDAWNELK GTSKEDAMKAYINKVEELKK KYGI
Tissue Specificity Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.
Function Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Protein Name Acyl-Coa-Binding Protein
Acbp
Diazepam-Binding Inhibitor
Dbi
Endozepine
Ep
Database Links Reactome: R-HSA-77289
Cellular Localisation Endoplasmic Reticulum
Golgi Apparatus
Golgi Localization Is Dependent On Ligand Binding
Alternative Antibody Names Anti-Acyl-Coa-Binding Protein antibody
Anti-Acbp antibody
Anti-Diazepam-Binding Inhibitor antibody
Anti-Dbi antibody
Anti-Endozepine antibody
Anti-Ep antibody
Anti-DBI antibody

Information sourced from Uniprot.org

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