• Western blot analysis of lysates from NIH/3T3 cells, using Keratin 14 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Keratin 14 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of NIH-3T3 cells using Cytokeratin 14/16 Polyclonal Antibody diluted at 1：1000
  • Immunofluorescence analysis of NIH/3T3 cells, using Keratin 14 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of 3T3 cells using Cytokeratin 14/16 Polyclonal Antibody diluted at 1：1000

Anti-Cytokeratin 14/16 antibody (1-50 aa) (STJ92627)

SKU:
STJ92627

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Keratin, type I cytoskeletal 14 and Keratin, type I cytoskeletal 16 (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT14
KRT16
Gene ID: 3861
3868
Uniprot ID: K1C14_HUMAN
K1C16_HUMAN
Immunogen Region: 1-50 aa
Specificity: Cytokeratin 14/16 Polyclonal Antibody detects endogenous levels of Cytokeratin 14/16 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Keratin 14 at the amino acid range 1-50
Post Translational Modifications A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Protein Name Keratin - Type I Cytoskeletal 14
Cytokeratin-14
Ck-14
Keratin-14
K14
Database Links Reactome: R-HSA-446107
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Expressed In Both As A Filamentous Pattern
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 14 antibody
Anti-Cytokeratin-14 antibody
Anti-Ck-14 antibody
Anti-Keratin-14 antibody
Anti-K14 antibody
Anti-KRT14 antibody

Information sourced from Uniprot.org

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