• Western blot analysis of extracts of MCF-7 cells, using CYP4B1 antibody (STJ28974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CYP4B1 antibody (30-250) (STJ28974)

SKU:
STJ28974

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYP4B1 (30-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP4B1
Gene ID: 1580
Uniprot ID: CP4B1_HUMAN
Immunogen Region: 30-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-250 of human CYP4B1 (NP_001093242.1).
Immunogen Sequence: IHLLLRRQTLAKAMDKFPGP PTHWLFGHALEIQETGSLDK VVSWAHQFPYAHPLWFGQFI GFLNIYEPDYAKAVYSRGDP KAPDVYDFFLQWIGRGLLVL EGPKWLQHRKLLTPGFHYDV LKPYVAVFTESTRIMLDKWE EKAREGKSFDIFCDVGHMAL NTLMKCTFGRGDTGLGHSRD SSYYLAVSDLTLLMQQRLVS FQYHNDFIYWLTPHGRRFLR A
Tissue Specificity Detected in the liver and lung (at protein level).
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Protein Name Cytochrome P450 4b1
Cypivb1
Cytochrome P450-Hp
Database Links Reactome: R-HSA-211935
Reactome: R-HSA-211958
Reactome: R-HSA-211979
Reactome: R-HSA-2142691
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 4b1 antibody
Anti-Cypivb1 antibody
Anti-Cytochrome P450-Hp antibody
Anti-CYP4B1 antibody

Information sourced from Uniprot.org

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