| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP27B1 (25-195) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP27B1 |
| Gene ID: |
1594 |
| Uniprot ID: |
CP27B_HUMAN |
| Immunogen Region: |
25-195 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 25-195 of human CYP27B1 (NP_000776.1). |
| Immunogen Sequence: |
LGYREYHSARRSLADIPGPS TPSFLAELFCKGGLSRLHEL QVQGAAHFGPVWLASFGTVR TVYVAAPALVEELLRQEGPR PERCSFSPWTEHRRCRQRAC GLLTAEGEEWQRLRSLLAPL LLRPQAAARYAGTLNNVVCD LVRRLRRQRGRGTGPPALVR DVAGEFYKFGL |
| Tissue Specificity | Kidney. |
| Function | A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial25-Ohd-1 Alpha-Hydroxylase25-Hydroxyvitamin D(3 1-Alpha-HydroxylaseVd3 1a HydroxylaseCalcidiol 1-MonooxygenaseCytochrome P450 Subfamily Xxviib Polypeptide 1Cytochrome P450c1 AlphaCytochrome P450vd1-AlphaCytochrome P450 27b1 |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-211916Reactome: R-HSA-5579014 |
| Cellular Localisation | Mitochondrion Membrane |
| Alternative Antibody Names | Anti-25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial antibodyAnti-25-Ohd-1 Alpha-Hydroxylase antibodyAnti-25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase antibodyAnti-Vd3 1a Hydroxylase antibodyAnti-Calcidiol 1-Monooxygenase antibodyAnti-Cytochrome P450 Subfamily Xxviib Polypeptide 1 antibodyAnti-Cytochrome P450c1 Alpha antibodyAnti-Cytochrome P450vd1-Alpha antibodyAnti-Cytochrome P450 27b1 antibodyAnti-CYP27B1 antibodyAnti-CYP1ALPHA antibodyAnti-CYP27B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
