| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP24A1 (36-448) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP24A1 |
| Gene ID: |
1591 |
| Uniprot ID: |
CP24A_HUMAN |
| Immunogen Region: |
36-448 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 36-448 of human CYP24A1 (NP_001122387.1). |
| Immunogen Sequence: |
PQPREVPVCPLTAGGETQNA AALPGPTSWPLLGSLLQILW KGGLKKQHDTLVEYHKKYGK IFRMKLGSFESVHLGSPCLL EALYRTESAYPQRLEIKPWK AYRDYRKEGYGLLILEGEDW QRVRSAFQKKLMKPGEVMKL DNKINEVLADFMGRIDELCD ERGHVEDLYSELNKWSFESI CLVLYEKRFGLLQKNAGDEA VNFIMAIKTMMSTFGRMMVT PVELHKSLNTKVWQDHTLA |
| Function | A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 1 -25-Dihydroxyvitamin D(3 24-Hydroxylase - Mitochondrial24-OhaseVitamin D(3 24-HydroxylaseCytochrome P450 24a1Cytochrome P450-Cc24 |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-211916Reactome: R-HSA-5579010 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-1 -25-Dihydroxyvitamin D(3 24-Hydroxylase - Mitochondrial antibodyAnti-24-Ohase antibodyAnti-Vitamin D(3 24-Hydroxylase antibodyAnti-Cytochrome P450 24a1 antibodyAnti-Cytochrome P450-Cc24 antibodyAnti-CYP24A1 antibodyAnti-CYP24 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
