• Western blot analysis of extracts of K-562 cells, using Cyclin E1 antibody (STJ11102739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human placenta using Cyclin E1 rabbit monoclonal antibody (STJ11102739) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Cyclin E1 rabbit monoclonal antibody (STJ11102739) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Cyclin E1 rabbit monoclonal antibody (STJ11102739) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Cyclin E1 rabbit monoclonal antibody (STJ11102739) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Cyclin E1 antibody (300-400) [S9MR] (STJ11102739)

SKU:
STJ11102739

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cyclin E1 (300-400) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNE1
Gene ID: 898
Uniprot ID: CCNE1_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Cyclin E1 (NP_001229.1).
Immunogen Sequence: AASALYHFSSSELMQKVSGY QWCDIENCVKWMVPFAMVIR ETGSSKLKHFRGVADEDAHN IQTHRDSLDLLDKARAKKAM LSEQNRASPLPSGLLTPPQS G
Tissue Specificity Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Post Translational Modifications Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2.appears to occur independently of phosphorylation.
Function Essential for the control of the cell cycle at the G1/S (start) transition.
Protein Name G1/S-Specific Cyclin-E1
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-187577
Reactome: R-HSA-2559586
Reactome: R-HSA-390471
Reactome: R-HSA-6804116
Reactome: R-HSA-69017
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-8849470
Reactome: R-HSA-9661069
Reactome: R-HSA-9706019
Cellular Localisation Nucleus
Alternative Antibody Names Anti-G1/S-Specific Cyclin-E1 antibody
Anti-CCNE1 antibody
Anti-CCNE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance