| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Human/Monkey/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-CX3CL1 (350-395) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5 µg/µl |
| Dilution Range: | WB: 1:500 ELISA: 1:10, 000 IP: 1:150 IHC: 1:50-1:100 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Cx3cl1 |
| Gene ID: | 20312 |
| Uniprot ID: | X3CL1_MOUSE |
| Immunogen Region: | 350-395 |
| Immunogen: | Synthetic peptide taken within amino acid reigon 350-395 on mouse Fractalkine protein. |
| Tissue Specificity | Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis. Highly expressed in lesional smooth muscle cells, but not macrophages. Low levels of ABCD-3 mRNA were also found in anti-CD40-stimulated splenic B-cells, but not in resting B-cells. Also expressed in dendritic cells. |
| Post Translational Modifications | A soluble short 80 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. |
| Function | Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. Processed fractalkine: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. Fractalkine: The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. |
| Protein Name | Fractalkine Fk Abcd-3 C-X3-C Motif Chemokine 1 Cx3c Membrane-Anchored Chemokine Neurotactin Small-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine |
| Database Links | Reactome: R-MMU-380108 Reactome: -MMU-418594 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Processed Fractalkine: Secreted |
| Alternative Antibody Names | Anti-Fractalkine antibody Anti-Fk antibody Anti-Abcd-3 antibody Anti-C-X3-C Motif Chemokine 1 antibody Anti-Cx3c Membrane-Anchored Chemokine antibody Anti-Neurotactin antibody Anti-Small-Inducible Cytokine D1 Cleaved Into - Processed Fractalkine antibody Anti-Cx3cl1 antibody Anti-Cx3c antibody Anti-Fkn antibody Anti-Scyd1 antibody |
Information sourced from Uniprot.org