• Immunohistochemistry analysis of paraffin-embedded human gastric cancer using CTSS antibody (STJ115443) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human mammary gland using CTSS antibody (STJ115443) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat brain, using CTSS antibody (STJ115443) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using CTSS antibody (STJ115443) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CTSS antibody (115-331) (STJ115443)

SKU:
STJ115443

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTSS (115-331) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSS
Gene ID: 1520
Uniprot ID: CATS_HUMAN
Immunogen Region: 115-331
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 115-331 of human CTSS (NP_004070.3).
Immunogen Sequence: LPDSVDWREKGCVTEVKYQG SCGACWAFSAVGALEAQLKL KTGKLVSLSAQNLVDCSTEK YGNKGCNGGFMTTAFQYIID NKGIDSDASYPYKAMDQKCQ YDSKYRAATCSKYTELPYGR EDVLKEAVANKGPVSVGVDA RHPSFFLYRSGVYYEPSCTQ NVNHGVLVVGYGDLNGKEYW LVKNSWGHNFGEEGYIRMAR NKGNHCGIASFPSYPEI
Function Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Protein Name Cathepsin S
Database Links Reactome: R-HSA-1236977
Reactome: R-HSA-1474228
Reactome: R-HSA-1679131
Reactome: R-HSA-2022090
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Secreted
Cytoplasmic Vesicle
Phagosome
Alternative Antibody Names Anti-Cathepsin S antibody
Anti-CTSS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance