| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CREB3L2/BBF2H7 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000. WB: Approx 55kDa band observed in Mouse and Rat Testis lysates (calculated MW of 57.4kDa according to Mouse NP_848776.2 and Rat NP_001012188.1). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CREB3L2 |
| Gene ID: | 64764 |
| Uniprot ID: | CR3L2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_919047.2 |
| Immunogen Sequence: | HSLQEPYTASVVRS |
| Post Translational Modifications | Upon ER stress, translocated to the Golgi apparatus, where it is processed by regulated intramembrane proteolysis (RIP) to release the cytosol-facing N-terminal transcription factor domain. The cleavage is performed sequentially by site-1 and site-2 proteases (S1P/MBTPS1 and S2P/MBTPS2). N-glycosylated. Ubiquitinated by HRD1/SYVN1.undergoes 'Lys-48'-linked ubiquitination, followed by rapid proteasomal degradation under normal conditions. Upon ER stress, SYVN1 E3 ubiquitin-protein ligase dissociates from its substrate, ubiquitination does not occur and CREB3L2 is stabilized. |
| Function | Transcription factor involved in unfolded protein response (UPR). In the absence of endoplasmic reticulum (ER) stress, inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. In response to ER stress, transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. Plays a critical role in chondrogenesis by activating the transcription of SEC23A, which promotes the transport and secretion of cartilage matrix proteins, and possibly that of ER biogenesis-related genes. In a neuroblastoma cell line, protects cells from ER stress-induced death. In vitro activates transcription of target genes via direct binding to the CRE site. |
| Protein Name | Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2 Camp-Responsive Element-Binding Protein 3-Like Protein 2 Bbf2 Human Homolog On Chromosome 7 Cleaved Into - Processed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2 |
| Database Links | Reactome: R-HSA-8874211 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type Ii Membrane Protein Er Membrane Resident Protein Upon Er Stress Translocated To The Golgi Apparatus Where It Is Cleaved The Cytosolic N-Terminal Fragment (Processed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 1) Is Transported Into The Nucleus Processed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2: Nucleus Translocated Into The Nucleus |
| Alternative Antibody Names | Anti-Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2 antibody Anti-Camp-Responsive Element-Binding Protein 3-Like Protein 2 antibody Anti-Bbf2 Human Homolog On Chromosome 7 Cleaved Into - Processed Cyclic Amp-Responsive Element-Binding Protein 3-Like Protein 2 antibody Anti-CREB3L2 antibody Anti-BBF2H7 antibody |
Information sourced from Uniprot.org