• Western blot analysis of extracts of various cell lines, using CPS1 antibody (STJ110382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using CPS1 Rabbit polyclonal antibody antibody (STJ110382) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HeLa cells using CPS1 Polyclonal Antibody (STJ110382) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CPS1 antibody (750-970) (STJ110382)

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STJ110382

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CPS1 (750-970) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPS1
Gene ID: 1373
Uniprot ID: CPSM_HUMAN
Immunogen Region: 750-970
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 750-970 of human CPS1 (NP_001866.2).
Immunogen Sequence: IKNVVSGKTSACFEPSLDYM VTKIPRWDLDRFHGTSSRIG SSMKSVGEVMAIGRTFEESF QKALRMCHPSIEGFTPRLPM NKEWPSNLDLRKELSEPSST RIYAIAKAIDDNMSLDEIEK LTYIDKWFLYKMRDILNMEK TLKGLNSESMTEETLKRAKE IGFSDKQISKCLGLTEAQTR ELRLKKNIHPWVKQIDTLAA EYPSVTNYLYVTYNGQEHDV N
Tissue Specificity Primarily in the liver and small intestine.
Post Translational Modifications Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus. Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation. Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation.
Function Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
Protein Name Carbamoyl-Phosphate Synthase Ammonia - Mitochondrial
Carbamoyl-Phosphate Synthetase I
Cpsase I
Database Links Reactome: R-HSA-70635
Cellular Localisation Mitochondrion
Nucleus
Nucleolus
Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Localizes To The Cell Surface Of Hepatocytes
Alternative Antibody Names Anti-Carbamoyl-Phosphate Synthase Ammonia - Mitochondrial antibody
Anti-Carbamoyl-Phosphate Synthetase I antibody
Anti-Cpsase I antibody
Anti-CPS1 antibody

Information sourced from Uniprot.org

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