Anti-COX2/PTGS2 antibody (C-Term) (STJ73734)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-COX2/PTGS2 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/IF/FC |
| Host | Goat |
| Reactivity | Human/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75-80kDa band observed in lysates of cell lines A549 and Daudi (predicted MW of 69kDa according to NP_000954.1). This molecular weight is routinely observed by other sources. Re |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PTGS2 |
| Gene ID | 5743 |
| Uniprot ID | PGH2_HUMAN |
| Accession Number | NP_000954.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | NPTVLLKERSTEL |
Additional Info
| Function | Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia. |
| Protein Name | Prostaglandin G/H Synthase 2Cyclooxygenase-2Cox-2Phs IiProstaglandin H2 Synthase 2Pgh Synthase 2Pghs-2Prostaglandin-Endoperoxide Synthase 2 |
| Database Links | Reactome: R-HSA-197264Reactome: R-HSA-2142770Reactome: R-HSA-2162123Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9018677Reactome: R-HSA-9018679Reactome: R-HSA-9025094Reactome: R-HSA-9027604 |
| Cellular Localisation | Microsome MembranePeripheral Membrane ProteinEndoplasmic Reticulum MembraneNucleus Inner MembraneNucleus Outer MembraneDetected On The Lumenal Side Of The Endoplasmic Reticulum And Nuclear Envelope |
| Alternative Antibody Names | Anti-Prostaglandin G/H Synthase 2 antibodyAnti-Cyclooxygenase-2 antibodyAnti-Cox-2 antibodyAnti-Phs Ii antibodyAnti-Prostaglandin H2 Synthase 2 antibodyAnti-Pgh Synthase 2 antibodyAnti-Pghs-2 antibodyAnti-Prostaglandin-Endoperoxide Synthase 2 antibodyAnti-PTGS2 antibodyAnti-COX2 antibody |
Information sourced from Uniprot.org