• Western blot analysis of extracts of SW480 cells, using CORO1C antibody (STJ23202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CORO1C antibody (375-474) (STJ23202)

SKU:
STJ23202

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CORO1C (375-474) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CORO1C
Gene ID: 23603
Uniprot ID: COR1C_HUMAN
Immunogen Region: 375-474
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 375-474 of human CORO1C (NP_055140.1).
Immunogen Sequence: EEWFEGKNADPILISLKHGY IPGKNRDLKVVKKNILDSKP TANKKCDLISIPKKTTDTAS VQNEAKLDEILKEIKSIKDT ICNQDERISKLEQQMAKIAA
Tissue Specificity Ubiquitous.
Function Plays a role in directed cell migration by regulating the activation and subcellular location of RAC1. Increases the presence of activated RAC1 at the leading edge of migrating cells. Required for normal organization of the cytoskeleton, including the actin cytoskeleton, microtubules and the vimentin intermediate filaments. Plays a role in endoplasmic reticulum-associated endosome fission: localizes to endosome membrane tubules and promotes recruitment of TMCC1, leading to recruitment of the endoplasmic reticulum to endosome tubules for fission. Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane. Required for normal cell proliferation, cell migration, and normal formation of lamellipodia. Required for normal distribution of mitochondria within cells. Isoform 3: Involved in myogenic differentiation.
Protein Name Coronin-1c
Coronin-3
Hcrnn4
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Lamellipodium
Ruffle Membrane
Cytoplasm
Cytoskeleton
Cell Cortex
Endosome Membrane
All Isoforms Colocalize With The Actin Cytoskeleton In The Cytosol
And Especially In The Cell Cortex
Colocalizes With F-Actin At The Leading Edge Of Lamellipodia
Partially Colocalizes With Microtubules And Vimentin Intermediate Filaments
Localizes To Endosome Membrane Tubules/Buds
Isoform 3: Cell Membrane
Sarcolemma
Myofibril
Sarcomere
Synapse
Colocalizes With The Thin Filaments Of The Sarcomere And With The Postsynaptic Area And The Junctional Sarcoplasm Of Motor End Plates
Colocalizes With The Actin Cytoskeleton In The Cytosol
Alternative Antibody Names Anti-Coronin-1c antibody
Anti-Coronin-3 antibody
Anti-Hcrnn4 antibody
Anti-CORO1C antibody
Anti-CRN2 antibody
Anti-CRNN4 antibody

Information sourced from Uniprot.org

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