• Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g COMT antibody (STJ23197). Western blot was performed from the immunoprecipitate using COMT antibody (STJ23197) at a dilution of 1:1000.
  • Western blot analysis of various lysates using COMT Rabbit polyclonal antibody (STJ23197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-COMT antibody (42-221) (STJ23197)

SKU:
STJ23197

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COMT (42-221) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COMT
Gene ID: 1312
Uniprot ID: COMT_HUMAN
Immunogen Region: 42-221
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
Immunogen Sequence: VGDKKGKIVDAVIQEHQPSV LLELGAYCGYSAVRMARLLS PGARLITIEINPDCAAITQR MVDFAGVKDKVTLVVGASQD IIPQLKKKYDVDTLDMVFLD HWKDRYLPDTLLLEECGLLR KGTVLLADNVICPGAPDFLA HVRGSSCFECTHYQSFLEYR EVVDGLEKAIYKGPGSEAGP
Tissue Specificity Brain, liver, placenta, lymphocytes and erythrocytes.
Post Translational Modifications The N-terminus is blocked.
Function Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Protein Name Catechol O-Methyltransferase
Database Links Reactome: R-HSA-156581
Reactome: R-HSA-379397
Reactome: R-HSA-379398
Reactome: R-HSA-9679191
Cellular Localisation Isoform Soluble: Cytoplasm
Isoform Membrane-Bound: Cell Membrane
Single-Pass Type Ii Membrane Protein
Extracellular Side
Alternative Antibody Names Anti-Catechol O-Methyltransferase antibody
Anti-COMT antibody

Information sourced from Uniprot.org

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