• Western blot analysis of lysates from 293 cells, using CLK1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HUVEC cells, using CLK1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of KB lysis using CLK1 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using CLK1 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CLK1 antibody (101-150 aa) (STJ92336)

SKU:
STJ92336

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Dual specificity protein kinase CLK1 (101-150 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLK1
Gene ID: 1195
Uniprot ID: CLK1_HUMAN
Immunogen Region: 101-150 aa
Specificity: CLK1 Polyclonal Antibody detects endogenous levels of CLK1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CLK1 at the amino acid range 101-150
Post Translational Modifications Autophosphorylates on all three types of residues.
Function Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Protein Name Dual Specificity Protein Kinase Clk1
Cdc-Like Kinase 1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dual Specificity Protein Kinase Clk1 antibody
Anti-Cdc-Like Kinase 1 antibody
Anti-CLK1 antibody
Anti-CLK antibody

Information sourced from Uniprot.org

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