• Western blot analysis of extracts of various cell lines, using CKAP2 antibody (STJ116368) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CKAP2 antibody (335-460) (STJ116368)

SKU:
STJ116368

$228.83 - $606.83
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CKAP2 (335-460) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CKAP2
Gene ID: 26586
Uniprot ID: CKAP2_HUMAN
Immunogen Region: 335-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 335-460 of human CKAP2 (NP_001273616.1).
Immunogen Sequence: EKSEPVDQRRHTAGKAIVDS RSAQPKETSEERKARLSEWK AGKGRVLKRPPNSVVTQHEP AGQNEKPVGSFWTTMAEEDE QRLFTEKVNNTFSECLNLIN EGCPKEDILVTLNDLIKNIP DAKKLV
Tissue Specificity Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
Function Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
Protein Name Cytoskeleton-Associated Protein 2
Ctcl Tumor Antigen Se20-10
Tumor- And Microtubule-Associated Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Spindle Pole
Contrary To The Ectopically Expressed Protein
Endogenous Ckap2 Does Not Colocalize With Microtubules In G1
S And Early G2
At Late G2 And Prophase After Separation Of Duplicated Centrosomes
Colocalizes With Gamma-Tubulin And Centrosome-Proximal Microtubules
From Prometaphase Through Anaphase B
Colocalizes With Mitotic Spindle Poles And Spindle Microtubules
During Cytokinesis
Absent From Midbody Microtubules
Alternative Antibody Names Anti-Cytoskeleton-Associated Protein 2 antibody
Anti-Ctcl Tumor Antigen Se20-10 antibody
Anti-Tumor- And Microtubule-Associated Protein antibody
Anti-CKAP2 antibody
Anti-LB1 antibody
Anti-TMAP antibody

Information sourced from Uniprot.org

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