• Western blot analysis of lysates from HT-29 cells, using CHST5 Rabbit polyclonal antibody (STJ119716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CHST5 antibody (232-411) (STJ119716)

SKU:
STJ119716

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHST5 (232-411) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHST5
Gene ID: 23563
Uniprot ID: CHST5_HUMAN
Immunogen Region: 232-411
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 232-411 of human CHST5 (NP_078809.2).
Immunogen Sequence: SREAAGPILARDNGIVLGTN GKWVEADPHLRLIREVCRSH VRIAEAATLKPPPFLRGRYR LVRFEDLAREPLAEIRALYA FTGLTLTPQLEAWIHNITHG SGIGKPIEAFHTSSRNARNV SQAWRHALPFTKILRVQEVC AGALQLLGYRPVYSADQQRD LTLDLVLPRGPDHFSWASPD
Tissue Specificity Predominantly expressed in small and large intestines and colon. Weakly expressed in lymphocytes. Not expressed in other tissues. Down-regulated in colonic adenocarcinomas.
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location.
Protein Name Carbohydrate Sulfotransferase 5
Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 4-Alpha
Gst4-Alpha
Intestinal N-Acetylglucosamine-6-O-Sulfotransferase
I-Glcnac6st
Intestinal Glcnac-6-Sulfotransferase
Hign6st
N-Acetylglucosamine 6-O-Sulfotransferase 3
Glcnac6st-3
Gn6st-3
Database Links Reactome: R-HSA-2022854
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Golgi Membrane
Early Secretory Pathway
Alternative Antibody Names Anti-Carbohydrate Sulfotransferase 5 antibody
Anti-Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 4-Alpha antibody
Anti-Gst4-Alpha antibody
Anti-Intestinal N-Acetylglucosamine-6-O-Sulfotransferase antibody
Anti-I-Glcnac6st antibody
Anti-Intestinal Glcnac-6-Sulfotransferase antibody
Anti-Hign6st antibody
Anti-N-Acetylglucosamine 6-O-Sulfotransferase 3 antibody
Anti-Glcnac6st-3 antibody
Anti-Gn6st-3 antibody
Anti-CHST5 antibody

Information sourced from Uniprot.org

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