| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CHST15 |
| Gene ID: | 51363 |
| Uniprot ID: | CHSTF_HUMAN |
| Immunogen Region: | 452-561 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 452-561 of human CHST15 (NP_056976.2). |
| Immunogen Sequence: | LQVGLYAVYLLDWLSVFDKQ QFLILRLEDHASNVKYTMHK VFQFLNLGPLSEKQEALMTK SPASNARRPEDRNLGPMWPI TQKILRDFYRPFNARLAQVL ADEAFAWKTT |
| Tissue Specificity | Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. |
| Post Translational Modifications | Glycosylated. |
| Function | Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression.however such results are unclear in vivo. |
| Protein Name | Carbohydrate Sulfotransferase 15 B-Cell Rag-Associated Gene Protein Hbrag N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase Galnac4s-6st |
| Database Links | Reactome: R-HSA-2022870 |
| Cellular Localisation | Golgi Apparatus Membrane Single-Pass Type Ii Membrane Protein A Small Fraction May Also Be Present At The Cell Surface Where It Acts As A B-Cell Receptor |
| Alternative Antibody Names | Anti-Carbohydrate Sulfotransferase 15 antibody Anti-B-Cell Rag-Associated Gene Protein antibody Anti-Hbrag antibody Anti-N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase antibody Anti-Galnac4s-6st antibody Anti-CHST15 antibody Anti-BRAG antibody Anti-GALNAC4S6ST antibody Anti-KIAA0598 antibody |
Information sourced from Uniprot.org