| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CHST10 (30-250) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHST10 |
| Gene ID: |
9486 |
| Uniprot ID: |
CHSTA_HUMAN |
| Immunogen Region: |
30-250 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 30-250 of human CHST10 (NP_004845.1). |
| Immunogen Sequence: |
KDPDVYSAKQEFLFLTTMPE VRKLPEEKHIPEELKPTGKE LPDSQLVQPLVYMERLELIR NVCRDDALKNLSHTPVSKFV LDRIFVCDKHKILFCQTPKV GNTQWKKVLIVLNGAFSSIE EIPENVVHDHEKNGLPRLSS FSDAEIQKRLKTYFKFFIVR DPFERLISAFKDKFVHNPRF EPWYRHEIAPGIIRKYRRNR TETRGIQFEDFVRYLGDPNH R |
| Tissue Specificity | In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe. |
| Function | Catalyzes the transfer of sulfate from 3'-phosphoadenylyl sulfate (PAPS) to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure 3-O-sulfo-beta-D-GlcA-(1->3)-beta-D-Gal-(1->4)-D-GlcNAc-R, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis. Sulfates terminal glucuronyl residue of the laminin globular (LG)-domain binding epitope on DAG1/alpha-dystroglycan and prevents further polymerization by LARGE1 glycosyltransferase. Likely defines the chain length of LG epitope, confering binding specificity to extracellular matrix components. Plays a role in down-regulating the steroid hormones. Sulfates glucuronidated estrogens and androgens with an impact in hormone cycle and fertility. Has a preference for glucuronyl moiety at the 3-hydroxyl group of a sterol ring rather than the 17-hydroxyl group, showing high catalytic efficiency for 17beta-estradiol 3-O-(beta-D-glucuronate) and dehydroepiandrosterone 3-O-(beta-D-glucuronate) hormones. |
| Protein Name | Carbohydrate Sulfotransferase 10Hnk-1 SulfotransferaseHnk-1stHnk1stHuhnk-1st |
| Database Links | Reactome: R-HSA-975578 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Carbohydrate Sulfotransferase 10 antibodyAnti-Hnk-1 Sulfotransferase antibodyAnti-Hnk-1st antibodyAnti-Hnk1st antibodyAnti-Huhnk-1st antibodyAnti-CHST10 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
