| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CHST1 (62-411) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CHST1 |
| Gene ID: |
8534 |
| Uniprot ID: |
CHST1_HUMAN |
| Immunogen Region: |
62-411 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 62-411 of human CHST1 (NP_003645.1). |
| Immunogen Sequence: |
HILILATTRSGSSFVGQLFN QHLDVFYLFEPLYHVQNTLI PRFTQGKSPADRRVMLGASR DLLRSLYDCDLYFLENYIKP PPVNHTTDRIFRRGASRVLC SRPVCDPPGPADLVLEEGDC VRKCGLLNLTVAAEACRERS HVAIKTVRVPEVNDLRALVE DPRLNLKVIQLVRDPRGILA SRSETFRDTYRLWRLWYGTG RKPYNLDVTQLTTVCEDFSN SVSTGLMRPPWLKGKYMLV |
| Tissue Specificity | Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes. |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of internal galactose (Gal) residues of keratan. Cooperates with B4GALT4 and B3GNT7 glycosyltransferases and CHST6 sulfotransferase to construct and elongate disulfated disaccharide unit ->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1-> within keratan sulfate polymer. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. Involved in biosynthesis of phosphacan, a major keratan sulfate proteoglycan in the developing brain. Involved in biosynthesis of 6-sulfoGalbeta-containing O-linked glycans in high endothelial venules of lymph nodes. May act in a synergistic manner with CHST4 to generate sialyl 6',6-disulfo Lewis X motif, a recognition determinant for immune cell receptors implicated in leukocyte trafficking. Catalyzes sulfation of N-acetyllactosamine (LacNAc) oligosaccharides with highest efficiency for sialylated LacNAc structures. |
| Protein Name | Carbohydrate Sulfotransferase 1Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 1Gst-1Keratan Sulfate Gal-6 SulfotransferaseKs6stKsgal6stKsst |
| Database Links | Reactome: R-HSA-2022854 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Carbohydrate Sulfotransferase 1 antibodyAnti-Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 1 antibodyAnti-Gst-1 antibodyAnti-Keratan Sulfate Gal-6 Sulfotransferase antibodyAnti-Ks6st antibodyAnti-Ksgal6st antibodyAnti-Ksst antibodyAnti-CHST1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
