• Western blot analysis of extracts of SH-SY5Y cells, using CHAT antibody (STJ11101569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Confocal imaging of paraffin-embedded mouse brain using CHAT rabbit monoclonal antibody (STJ11101569, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
  • Confocal imaging of paraffin-embedded rat brain using CHAT rabbit monoclonal antibody (STJ11101569, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

Anti-CHAT antibody (649-748) [S9MR] (STJ11101569)

SKU:
STJ11101569

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CHAT (649-748) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAT
Gene ID: 1103
Uniprot ID: CLAT_HUMAN
Immunogen Region: 649-748
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 649-748 of human CHAT (P28329).
Immunogen Sequence: MSNRFVLSTSQVPTTTEMFC CYGPVVPNGYGACYNPQPET ILFCISSFHSCKETSSSKFA KAVEESLIDMRDLCSLLPPT ESKPLATKEKATRPSQGHQP
Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Protein Name Choline O-Acetyltransferase
Choactase
Chat
Choline Acetylase
Database Links Reactome: R-HSA-1483191
Reactome: R-HSA-264642
Alternative Antibody Names Anti-Choline O-Acetyltransferase antibody
Anti-Choactase antibody
Anti-Chat antibody
Anti-Choline Acetylase antibody
Anti-CHAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance