• Immunofluorescence analysis of HeLa cells using CEP85 Rabbit polyclonal antibody (STJ118336) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CEP85 Rabbit polyclonal antibody (STJ118336) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CEP85 Rabbit polyclonal antibody (STJ118336) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CEP85 antibody (130-430) (STJ118336)

SKU:
STJ118336

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CEP85 (130-430) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CEP85
Gene ID: 64793
Uniprot ID: CEP85_HUMAN
Immunogen Region: 130-430
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-430 of human CEP85 (NP_073615.2).
Immunogen Sequence: TRNGDLGAMKHSPGLSRDLM YFSGATGENGIEQSWFPAVG HERQEEARKFDIPSMESTLN QSAMMETLYSDPHHRVRFHN PRTSTSKELYRVLPEAKKAP GSGAVFERNGPHSNSSGVLP LGLQPAPGLSKPLPSQVWQP SPDTWHPREQSCELSTCRQQ LELIRLQMEQMQLQNGAICH HPAAFGPSLPILEPAQWISI LNSNEHLLKEKELLIDKQRK HISQLEQKVRESELQVHSA
Function Acts as a negative regulator of NEK2 to maintain the centrosome integrity in interphase. Suppresses centrosome disjunction by inhibiting NEK2 kinase activity.
Protein Name Centrosomal Protein Of 85 Kda
Cep85
Coiled-Coil Domain-Containing Protein 21
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Nucleus
Nucleolus
Localizes To Centrosomes And Nucleolus In Interphase
Upon Entry Into Mitosis
Relocates From Nucleolus And Accumulates At Spindle Poles
Associated With The Pericentriolar Material
Localizes To Centrosomes At A Low Level In G1 Phase And A Slightly Increased Level In S Phase
With Gradually Elevated Levels During G2 Phase
The Levels At Centrosomes Further Increase At G2/M
Reaching A Peak At Spindle Poles At Early Mitotic Stages And Remain High Until The End Of Anaphase
Alternative Antibody Names Anti-Centrosomal Protein Of 85 Kda antibody
Anti-Cep85 antibody
Anti-Coiled-Coil Domain-Containing Protein 21 antibody
Anti-CEP85 antibody
Anti-CCDC21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance