• Western blot analysis of extracts of 293T cells, using CEP27 antibody (STJ115661) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CEP27 antibody (1-235) (STJ115661)

SKU:
STJ115661

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CEP27 (1-235) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAUS2
Gene ID: 55142
Uniprot ID: HAUS2_HUMAN
Immunogen Region: 1-235
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human CEP27 (NP_060567.1).
Immunogen Sequence: MAAANPWDPASAPNGAGLVL GHFIASGMVNQEMLNMSKKT VSCFVNFTRLQQITNIQAEI YQKNLEIELLKLEKDTADVV HPFFLAQKCHTLQSMNNHLE AVLKEKRSLRQRLLKPMCQE NLPIEAVYHRYMVHLLELAV TFIERLETHLETIRNIPHLA ANLKKMNQALAKMDILVTET EELAENILKWRKQQNEVSSC IPKILAEESYLYKHDIIMPP LPFTSKVHVQTINAK
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Protein Name Haus Augmin-Like Complex Subunit 2
Centrosomal Protein Of 27 Kda
Cep27
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Localizes To Interphase Centrosomes And To Mitotic Spindle Microtubules
Alternative Antibody Names Anti-Haus Augmin-Like Complex Subunit 2 antibody
Anti-Centrosomal Protein Of 27 Kda antibody
Anti-Cep27 antibody
Anti-HAUS2 antibody
Anti-C15orf25 antibody
Anti-CEP27 antibody

Information sourced from Uniprot.org

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