• Immunofluorescence analysis of HeLa cells using CDKN2C/p18-INK4C antibody (STJ11104285). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using CDKN2C/p18-INK4C antibody (STJ11104285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using CDKN2C/p18-INK4C antibody (STJ11104285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using CDKN2C/p18-INK4C antibody (STJ11104285) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CDKN2C/p18-INK4C antibody (STJ11104285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CDKN2C antibody (1-168) (STJ11104285)

SKU:
STJ11104285

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cyclin-Dependent Kinase 4 Inhibitor C (1-168) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDKN2C
Gene ID: 1031
Uniprot ID: CDN2C_HUMAN
Immunogen Region: 1-168
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human CDKN2C/p18-INK4C (NP_523240.1).
Immunogen Sequence: MAEPWGNELASAAARGDLEQ LTSLLQNNVNVNAQNGFGRT ALQVMKLGNPEIARRLLLRG ANPDLKDRTGFAVIHDAARA GFLDTLQTLLEFQADVNIED NEGNLPLHLAAKEGHLRVVE FLVKHTASNVGHRNHKGDTA CDLARLYGRNEVVSLMQANG AGGATNLQ
Tissue Specificity Highest levels found in skeletal muscle. Also found in pancreas and heart.
Function Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Protein Name Cyclin-Dependent Kinase 4 Inhibitor C
Cyclin-Dependent Kinase 6 Inhibitor
P18-Ink4c
P18-Ink6
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-69231
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 4 Inhibitor C antibody
Anti-Cyclin-Dependent Kinase 6 Inhibitor antibody
Anti-P18-Ink4c antibody
Anti-P18-Ink6 antibody
Anti-CDKN2C antibody
Anti-CDKN6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance