• Western blot analysis of extracts of various cell lines, using CDKN2A/p16INK4a antibody (STJ11100176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry of paraffin-embedded HeLa using CDKN2A/p16INK4a mouse monoclonal antibody (STJ11100176) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human cervix (negative control samples) using CDKN2A/p16INK4a mouse monoclonal antibody (STJ11100176) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using CDKN2A/p16INK4a mouse monoclonal antibody (STJ11100176) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDKN2A antibody [S6MM] (STJ11100176)

SKU:
STJ11100176

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Host: Mouse
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-CDKN2A/p16INK4a is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MM
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Tris Buffer, pH7.3-7.7, with 1% BSA and <0.1% Sodium Azide.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at 4°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDKN2A
CDKN2A
Gene ID: 1029
1029
Uniprot ID: CDN2A_HUMAN
ARF_HUMAN
Immunogen: Recombinant protein of human CDKN2A/p16INK4a.
Immunogen Sequence: Email for sequence
Tissue Specificity
Alternative Antibody Names Anti-Cyclin-Dependent Kinase Inhibitor 2a antibody
Anti-Cyclin-Dependent Kinase 4 Inhibitor A antibody
Anti-Cdk4i antibody
Anti-Multiple Tumor Suppressor 1 antibody
Anti-Mts-1 antibody
Anti-P16-Ink4a antibody
Anti-P16-Ink4 antibody
Anti-P16ink4a antibody
Anti-CDKN2A antibody
Anti-CDKN2 antibody
Anti-MTS1 antibody
Anti-Tumor Suppressor Arf antibody
Anti-Alternative Reading Frame antibody
Anti-Arf antibody
Anti-Cyclin-Dependent Kinase Inhibitor 2a antibody
Anti-P14arf antibody
Anti-CDKN2A antibody
Anti-CDKN2 antibody
Anti-MLM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance