• Immunofluorescence analysis of U-2 OS cells using CDKN1B/p27KIP1 Rabbit polyclonal antibody (STJ119133) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CDKN1B/p27KIP1 Rabbit polyclonal antibody (STJ119133) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CDKN1B/p27KIP1 in paraffin-embedded rat liver using CDKN1B/p27KIP1 Rabbit polyclonal antibody (STJ119133) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CDKN1B/p27KIP1 in paraffin-embedded human colon using CDKN1B/p27KIP1 Rabbit polyclonal antibody (STJ119133) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CDKN1B/p27KIP1 Rabbit polyclonal antibody (STJ119133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDKN1B antibody (1-198) (STJ119133)

SKU:
STJ119133

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDKN1B/p27KIP1 (1-198) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDKN1B
Gene ID: 1027
Uniprot ID: CDN1B_HUMAN
Immunogen Region: 1-198
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human CDKN1B/p27KIP1 (NP_004055.1).
Immunogen Sequence: MSNVRVSNGSPSLERMDARQ AEHPKPSACRNLFGPVDHEE LTRDLEKHCRDMEEASQRKW NFDFQNHKPLEGKYEWQEVE KGSLPEFYYRPPRPPKGACK VPAQESQDVSGSRPAAPLIG APANSEDTHLVDPKTDPSDS QTGLAEQCAGIRKRPATDDS STQNKRANRTEENVSDGSPN AGSVEQTPKKPGLRRRQT
Tissue Specificity Expressed in kidney (at protein level). Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Post Translational Modifications Phosphorylated.phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF. Ubiquitinated.in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated.by a TRIM21-containing SCF(SKP2)-like complex.leads to its degradation. Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation.
Function Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Protein Name Cyclin-Dependent Kinase Inhibitor 1b
Cyclin-Dependent Kinase Inhibitor P27
P27kip1
Database Links Reactome: R-HSA-187577
Reactome: R-HSA-198323
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-5625900
Reactome: R-HSA-5674400
Reactome: R-HSA-6804116
Reactome: R-HSA-69202
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-69656
Reactome: R-HSA-8849470
Reactome: R-HSA-9607240
Reactome: R-HSA-9617828
Reactome: R-HSA-9634638
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Cytoplasm
Endosome
Nuclear And Cytoplasmic In Quiescent Cells
Akt- Or Rsk-Mediated Phosphorylation On Thr-198
Binds 14-3-3
Translocates To The Cytoplasm And Promotes Cell Cycle Progression
Mitogen-Activated Uhmk1 Phosphorylation On Ser-10 Also Results In Translocation To The Cytoplasm And Cell Cycle Progression
Phosphorylation On Ser-10 Facilitates Nuclear Export
Translocates To The Nucleus On Phosphorylation Of Tyr-88 And Tyr-89
Colocalizes At The Endosome With Snx6
This Leads To Lysosomal Degradation
Alternative Antibody Names Anti-Cyclin-Dependent Kinase Inhibitor 1b antibody
Anti-Cyclin-Dependent Kinase Inhibitor P27 antibody
Anti-P27kip1 antibody
Anti-CDKN1B antibody
Anti-KIP1 antibody
Anti-p27 antibody

Information sourced from Uniprot.org

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