| Host: | Rabbit |
| Applications: | IHC-P |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal antibody anti-CDK4 is suitable for use in Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | EP180 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Tris Buffer, pH 7.3-7.7, containing 1% BSA and <0.1% Sodium Azide |
| Dilution Range: | IHC-P 1:100-1:200 |
| Storage Instruction: | Store at 2-8°C upon receipt. |
| Gene Symbol: | CDK4 |
| Gene ID: | 1019 |
| Uniprot ID: | CDK4_HUMAN |
| Immunogen: | a synthetic peptide corresponding to residues of human CDK4 protein |
| Post Translational Modifications | Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B. |
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Protein Name | Cyclin-Dependent Kinase 4 Cell Division Protein Kinase 4 Psk-J3 |
| Database Links | Reactome: R-HSA-187577 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559585 Reactome: R-HSA-3214858 Reactome: R-HSA-381340 Reactome: R-HSA-69231 Reactome: R-HSA-75815 Reactome: R-HSA-8849470 Reactome: R-HSA-8878166 Reactome: R-HSA-912446 Reactome: R-HSA-9616222 Reactome: R-HSA-9630791 Reactome: R-HSA-9630794 Reactome: R-HSA-9632697 Reactome: R-HSA-9632700 Reactome: R-HSA-9661069 Reactome: R-HSA-9754119 |
| Cellular Localisation | Cytoplasm Nucleus Nucleus Membrane Cytoplasmic When Non-Complexed Forms A Cyclin D-Cdk4 Complex In The Cytoplasm As Cells Progress Through G(1) Phase The Complex Accumulates On The Nuclear Membrane And Enters The Nucleus On Transition From G(1) To S Phase Also Present In Nucleoli And Heterochromatin Lumps Colocalizes With Rb1 After Release Into The Nucleus |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 4 antibody Anti-Cell Division Protein Kinase 4 antibody Anti-Psk-J3 antibody Anti-CDK4 antibody |
Information sourced from Uniprot.org