• Western blot analysis of various lysates, using CD86 Rabbit polyclonal antibody (STJ11105173) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD86 antibody (STJ11105173)

SKU:
STJ11105173

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD86 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cd86
Gene ID: 12524
Uniprot ID: CD86_MOUSE
Immunogen: Recombinant protein of mouse CD86.
Tissue Specificity Expressed on activated B-cells.
Post Translational Modifications Polyubiquitinated.which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.
Protein Name T-Lymphocyte Activation Antigen Cd86
Activation B7-2 Antigen
Early T-Cell Costimulatory Molecule 1
Etc-1
Cd Antigen Cd86
Database Links Reactome: R-MMU-1257604
Reactome: -MMU-389356
Reactome: -MMU-389357
Reactome: -MMU-389359
Reactome: -MMU-389513
Reactome: -MMU-6811558
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Lymphocyte Activation Antigen Cd86 antibody
Anti-Activation B7-2 Antigen antibody
Anti-Early T-Cell Costimulatory Molecule 1 antibody
Anti-Etc-1 antibody
Anti-Cd Antigen Cd86 antibody
Anti-Cd86 antibody

Information sourced from Uniprot.org

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