• Immunofluorescence was carried out on 4% PFA fixed Hepa1-6 cells, using Anti-CD63 polyclonal antibody at a 1:50 dilution.
  • Western Blot was carried out using Anti-CD63 polyclonal antibody at a 1:2,500 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
  • Immunofluorescence was carried out on 4% PFA fixed Hepa1-6 cells, using Anti-CD63 polyclonal antibody at a 1:50 dilution.
  • Western Blot was carried out using Anti-CD63 polyclonal antibody at a 1:2,500 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
  • Immunofluorescence was carried out on 4% PFA fixed Hepa1-6 cells, using Anti-CD63 polyclonal antibody at a 1:50 dilution.
  • Western Blot was carried out using Anti-CD63 polyclonal antibody at a 1:2,500 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.

Anti-CD63 antibody (STJ140029)

SKU:
STJ140029
Current Stock:
Host: Goat
Applications: WB, IF
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Donkey, Cat, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Goat polyclonal primary antibody against Lysosome-associated membrane glycoprotein 3.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS, 20% glycerol and 0.05% sodium azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Storage Instruction: For continuous use, store at 2-8 °C for one-two days. For extended storage, store in-20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Concentration: 3 mg/mL
Isotype: IgG
Specificity: Reacts with CD63, a 40-60 kDa glycoprotein, detected by western blot in the following human (HeLa, HUH, Jurkat) , mouse (AtT-20, Hepa, 3T3, RAW264.7) , canine (MDCK) and monkey (COS-7) whole cell lysates.
Gene ID: 967
Gene Symbol: CD63
Immunogen: Purified recombinant peptide derived from within residues 120 aa to 175 aa of human CD63 produced in E. coli.
Background CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of the members are cellsurface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.
Tissue Specificity Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
Post Translational Modifications Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).
Function Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Database Links Reactome: R-HSA-114608
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Lysosome Membrane
Late Endosome Membrane
Endosome
Multivesicular Body
Melanosome
Secreted
Extracellular Exosome
Cell Surface
Also Found In Weibel-Palade Bodies Of Endothelial Cells
Located In Platelet Dense Granules
Detected In A Subset Of Pre-Melanosomes
Detected On Intralumenal Vesicles (Ilvs) Within Multivesicular Bodies
Protein Name Cd63 Antigen
Granulophysin
Lysosomal-Associated Membrane Protein 3
Lamp-3
Melanoma-Associated Antigen Me491
Oma81h
Ocular Melanoma-Associated Antigen
Tetraspanin-30
Tspan-30
Cd Antigen Cd63
Alternative Names Anti-Cd63 Antigen antibody
Anti-Granulophysin antibody
Anti-Lysosomal-Associated Membrane Protein 3 antibody
Anti-Lamp-3 antibody
Anti-Melanoma-Associated Antigen Me491 antibody
Anti-Oma81h antibody
Anti-Ocular Melanoma-Associated Antigen antibody
Anti-Tetraspanin-30 antibody
Anti-Tspan-30 antibody
Anti-Cd Antigen Cd63 antibody
Anti-CD63 antibody
Anti-MLA1 antibody
Anti-TSPAN30 antibody
validated_applications WB, IF
gene_identifier ENSG00000135404

Information sourced from Uniprot.org

6 months standard