Home
Primary Antibodies
Polyclonal Antibodies
Anti-CD63 antibody (STJ140029)

Immunofluorescence was carried out on 4% PFA fixed Hepa1-6 cells, using Anti-CD63 polyclonal antibody at a 1:50 dilution.
Western Blot was carried out using Anti-CD63 polyclonal antibody at a 1:2,500 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
Immunofluorescence was carried out on 4% PFA fixed Hepa1-6 cells, using Anti-CD63 polyclonal antibody at a 1:50 dilution.
Western Blot was carried out using Anti-CD63 polyclonal antibody at a 1:2,500 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.
Immunofluorescence was carried out on 4% PFA fixed Hepa1-6 cells, using Anti-CD63 polyclonal antibody at a 1:50 dilution.
Western Blot was carried out using Anti-CD63 polyclonal antibody at a 1:2,500 dilution, with lysates of 50 µg per lane and secondary antibody rabbit anti-goat IgG (HRP) at 1:10,000 dilution.

Anti-CD63 antibody (STJ140029)

SKU:

STJ140029

£40.00 - £556.00

Current Stock:

General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Goat
Applications:	WB, IF
Reactivity:	Human, Mouse, Rat, Bovine, Canine, Chicken, Donkey, Cat, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
Note:	FOR RESEARCH USE ONLY (RUO).
Short Description:	Goat polyclonal primary antibody against Lysosome-associated membrane glycoprotein 3.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Formulation:	PBS, 20% glycerol and 0.05% sodium azide.
Purification:	This antibody is epitope-affinity purified from goat antiserum.
Storage Instruction:	For continuous use, store at 2-8 °C for one-two days. For extended storage, store in-20 °C freezer. Working dilution samples should be discarded if not used within 12 hours.
Concentration:	3 mg/mL
Isotype:	IgG

Specificity:	Reacts with CD63, a 40-60 kDa glycoprotein, detected by western blot in the following human (HeLa, HUH, Jurkat) , mouse (AtT-20, Hepa, 3T3, RAW264.7) , canine (MDCK) and monkey (COS-7) whole cell lysates.
Gene ID:	967
Gene Symbol:	CD63
Immunogen:	Purified recombinant peptide derived from within residues 120 aa to 175 aa of human CD63 produced in E. coli.

Background	CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of the members are cellsurface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.
Tissue Specificity	Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
Post Translational Modifications	Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro).
Function	Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Database Links	Reactome: R-HSA-114608
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Lysosome Membrane Late Endosome Membrane Endosome Multivesicular Body Melanosome Secreted Extracellular Exosome Cell Surface Also Found In Weibel-Palade Bodies Of Endothelial Cells Located In Platelet Dense Granules Detected In A Subset Of Pre-Melanosomes Detected On Intralumenal Vesicles (Ilvs) Within Multivesicular Bodies
Protein Name	Cd63 Antigen Granulophysin Lysosomal-Associated Membrane Protein 3 Lamp-3 Melanoma-Associated Antigen Me491 Oma81h Ocular Melanoma-Associated Antigen Tetraspanin-30 Tspan-30 Cd Antigen Cd63
Alternative Names	Anti-Cd63 Antigen antibody Anti-Granulophysin antibody Anti-Lysosomal-Associated Membrane Protein 3 antibody Anti-Lamp-3 antibody Anti-Melanoma-Associated Antigen Me491 antibody Anti-Oma81h antibody Anti-Ocular Melanoma-Associated Antigen antibody Anti-Tetraspanin-30 antibody Anti-Tspan-30 antibody Anti-Cd Antigen Cd63 antibody Anti-CD63 antibody Anti-MLA1 antibody Anti-TSPAN30 antibody
validated_applications	WB, IF
gene_identifier	ENSG00000135404

Information sourced from Uniprot.org

6 months standard

reviews