• Western blot analysis of extracts of Jurkat cells, using CD5 antibody (STJ11103664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Confocal imaging of Jurkat cells using CD5 Rabbit monoclonal antibody (STJ11103664, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human spleen using CD5 rabbit monoclonal antibody (STJ11103664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CD5 in paraffin-embedded human spleen using CD5 Rabbit monoclonal antibody (STJ11103664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Jurkat cells using CD5 rabbit monoclonal antibody (STJ11103664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Jurkat cells, using CD5 Rabbit monoclonal antibody (STJ11103664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD5 antibody (1-100) [S4MR] (STJ11103664)

SKU:
STJ11103664

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD5 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD5
Gene ID: 921
Uniprot ID: CD5_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD5 (P06127).
Immunogen Sequence: MPMGSLQPLATLYLLGMLVA SCLGRLSWYDPDFQARLTRS NSKCQGQLEVYLKDGWHMVC SQSWGRSSKQWEDPSQASKV CQRLNCGVPLSLGPFLVTYT
Post Translational Modifications Phosphorylated on tyrosine residues by LYN.this creates binding sites for PTPN6/SHP-1.
Function May act as a receptor in regulating T-cell proliferation.
Protein Name T-Cell Surface Glycoprotein Cd5
Lymphocyte Antigen T1/Leu-1
Cd Antigen Cd5
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd5 antibody
Anti-Lymphocyte Antigen T1/Leu-1 antibody
Anti-Cd Antigen Cd5 antibody
Anti-CD5 antibody
Anti-LEU1 antibody

Information sourced from Uniprot.org

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