• Western blot analysis of extracts of various cell lines, using CD4 antibody (STJ11101556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human liver using CD4 rabbit monoclonal antibody (STJ11101556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD4 rabbit monoclonal antibody (STJ11101556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Flow cytometry:1X10^6 K-562 cells (negative control, left) and THP-1 cells (right) were surface-stained with CD4 rabbit monoclonal antibody (STJ11101556, 10 Mu g/mL, orange line) or rabbit IgG isotype control (10 Mu g/mL, blue line) , followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:500 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
  • Anti-CD4 antibody (216-396) [S6MR] (STJ11101556)

Anti-CD4 antibody (216-396) [S6MR] (STJ11101556)

SKU:
STJ11101556

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CD4 (216-396) is suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
FC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD4
Gene ID: 920
Uniprot ID: CD4_HUMAN
Immunogen Region: 216-396
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 216-396 of human CD4 (P01730).
Immunogen Sequence: GEQVEFSFPLAFTVEKLTGS GELWWQAERASSSKSWITFD LKNKEVSVKRVTQDPKLQMG KKLPLHLTLPQALPQYAGSG NLTLALEAKTGKLHQEVNLV VMRATQLQKNLTCEVWGPTS PKLMLSLKLENKEAKVSKRE KAVWVLNPEAGMWQCLLSDS GQVLLESNIKVLPTWSTPVQ P
Tissue Specificity Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.
Post Translational Modifications Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation at Ser-433 plays an important role for CD4 internalization.
Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1). Down-regulated by HIV-1 Vpu. Acts as a receptor for Human Herpes virus 7/HHV-7.
Protein Name T-Cell Surface Glycoprotein Cd4
T-Cell Surface Antigen T4/Leu-3
Cd Antigen Cd4
Database Links Reactome: R-HSA-1462054
Reactome: R-HSA-167590
Reactome: R-HSA-173107
Reactome: R-HSA-180534
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-389948
Reactome: R-HSA-449836
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Localizes To Lipid Rafts
Removed From Plasma Membrane By Hiv-1 Nef Protein That Increases Clathrin-Dependent Endocytosis Of This Antigen To Target It To Lysosomal Degradation
Cell Surface Expression Is Also Down-Modulated By Hiv-1 Envelope Polyprotein Gp160 That Interacts With
And Sequesters Cd4 In The Endoplasmic Reticulum
Alternative Antibody Names Anti-T-Cell Surface Glycoprotein Cd4 antibody
Anti-T-Cell Surface Antigen T4/Leu-3 antibody
Anti-Cd Antigen Cd4 antibody
Anti-CD4 antibody

Information sourced from Uniprot.org

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