• Western blot analysis of extracts of various cell lines, using Cyclin A1 antibody (STJ27598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of Rat testis, using Cyclin A1 antibody (STJ27598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CCNA1 antibody (1-230) (STJ27598)

SKU:
STJ27598

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCNA1 (1-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNA1
Gene ID: 8900
Uniprot ID: CCNA1_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CCNA1 (NP_001104515.1).
Immunogen Sequence: METGFPAIMYPGSFIGGWGE EYLSWEGPGLPDFVFQQPVE SEAMHCSNPKSGVVLATVAR GPDACQILTRAPLGQDPPQR TVLGLLTANGQYRRTCGQGI TRIRCYSGSENAFPPAGKKA LPDCGVQEPPKQGFDIYMDE LEQGDRDSCSVREGMAFEDV YEVDTGTLKSDLHFLLDFNT VSPMLVDSSLLSQSEDISSL GTDVINVTEYAEEIYQYLRE AEIRHRPKAH
Tissue Specificity Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.
Post Translational Modifications Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
Function May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
Protein Name Cyclin-A1
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-170145
Reactome: R-HSA-171319
Reactome: R-HSA-174184
Reactome: R-HSA-176408
Reactome: R-HSA-187577
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-5689880
Reactome: R-HSA-5693607
Reactome: R-HSA-6804116
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-68911
Reactome: R-HSA-68949
Reactome: R-HSA-69017
Reactome: R-HSA-69205
Reactome: R-HSA-69273
Reactome: R-HSA-69563
Reactome: R-HSA-69656
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclin-A1 antibody
Anti-CCNA1 antibody

Information sourced from Uniprot.org

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