• Immunofluorescence analysis of U-2 OS cells using CCDC59 Rabbit polyclonal antibody (STJ116611) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using CCDC59 Rabbit polyclonal antibody (STJ116611) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CCDC59 in paraffin-embedded human lung cancer using CCDC59 Rabbit polyclonal antibody (STJ116611) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CCDC59 in paraffin-embedded rat testis using CCDC59 Rabbit polyclonal antibody (STJ116611) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CCDC59 Rabbit polyclonal antibody (STJ116611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-CCDC59 antibody (1-241) (STJ116611)

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STJ116611

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCDC59 (1-241) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCDC59
Gene ID: 29080
Uniprot ID: TAP26_HUMAN
Immunogen Region: 1-241
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human CCDC59 (NP_054886.2).
Immunogen Sequence: MAPVRRSAKWRPGGIEARGE GVSTVGYRNKNVRQKTWRPN HPQAFVGSVREGQGFAFRRK LKIQQSYKKLLRKEKKAQTS LESQFTDRYPDNLKHLYLAE EERHRKQARKVDHPLSEQVH QPLLEEQCSIDEPLFEDQCS FDQPQPEEQCIKTVNSFTIP KKNKKKTSNQKAQEEYEQIQ AKRAAKKQEFERRKQEREEA QRQYKKKKMEVFKILNKKTK KGQPNLNVQMEYLLQKIQE
Tissue Specificity Ubiquitously expressed. In lung, expression is restricted to the alveolar epithelial cells.
Function Component of the transcription complexes of the pulmonary surfactant-associated protein-B (SFTPB) and -C (SFTPC). Enhances homeobox protein Nkx-2.1-activated SFTPB and SFTPC promoter activities.
Protein Name Thyroid Transcription Factor 1-Associated Protein 26
Ttf-1-Associated Protein 26
Coiled-Coil Domain-Containing Protein 59
Ttf-1-Associated Protein Br2
Database Links Reactome: R-HSA-5683826
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Thyroid Transcription Factor 1-Associated Protein 26 antibody
Anti-Ttf-1-Associated Protein 26 antibody
Anti-Coiled-Coil Domain-Containing Protein 59 antibody
Anti-Ttf-1-Associated Protein Br2 antibody
Anti-CCDC59 antibody
Anti-BR22 antibody
Anti-TAP26 antibody
Anti-HSPC128 antibody

Information sourced from Uniprot.org

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