• Immunohistochemistry analysis of Caveolin-1 in paraffin-embedded Human lung using Caveolin-1 Rabbit monoclonal antibody (STJ11101544) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Caveolin-1 in paraffin-embedded Human colon using Caveolin-1 Rabbit monoclonal antibody (STJ11101544) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using Caveolin-1 Rabbit monoclonal antibody (STJ11101544) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates, using Caveolin-1 Rabbit monoclonal antibody (STJ11101544) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CAV1 antibody (1-80) [S4MR] (STJ11101544)

SKU:
STJ11101544

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Caveolin-1 (1-80) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAV1
Gene ID: 857
Uniprot ID: CAV1_HUMAN
Immunogen Region: 1-80
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human Caveolin-1 (NP_001744.2).
Immunogen Sequence: MSGGKYVDSEGHLYTVPIRE QGNIYKPNNKAMADELSEKQ VYDAHTKEIDLVNRDPKHLN DDVVKIDFEDVIAEPEGTHS
Tissue Specificity Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain.
Post Translational Modifications Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation. Ubiquitinated by ZNRF1.leading to degradation and modulation of the TLR4-mediated immune response. The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress.
Function May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation.
Protein Name Caveolin-1
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-203615
Reactome: R-HSA-203641
Reactome: R-HSA-210991
Reactome: R-HSA-4641262
Reactome: R-HSA-5218920
Reactome: R-HSA-8980692
Reactome: R-HSA-9009391
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013406
Reactome: R-HSA-9013407
Reactome: R-HSA-9013408
Reactome: R-HSA-9013409
Reactome: R-HSA-9013423
Reactome: R-HSA-9035034
Reactome: R-HSA-9617828
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cell Membrane
Membrane
Caveola
Membrane Raft
Golgi Apparatus
Trans-Golgi Network
Colocalized With Dpp4 In Membrane Rafts
Potential Hairpin-Like Structure In The Membrane
Membrane Protein Of Caveolae
Alternative Antibody Names Anti-Caveolin-1 antibody
Anti-CAV1 antibody
Anti-CAV antibody

Information sourced from Uniprot.org

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