| Host: | Rabbit |
| Applications: | IF/WB/IHC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Caspase-1 (350-400 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IF 1:50-200 WB 1:500-2000 IHC 1:50-300 ELISA 1:10000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CASP1 |
| Gene ID: | 834 |
| Uniprot ID: | CASP1_HUMAN |
| Immunogen Region: | 350-400 aa |
| Specificity: | Caspase-1 Polyclonal Antibody detects endogenous levels of Caspase-1 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the C-terminal region of human CASP1 at the amino acid range 350-400 |
| Post Translational Modifications | The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8. Cleavage in the interdomain linker region is required to induce pyroptosis. |
| Function | Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. Isoform Delta: Apoptosis inactive. Isoform Epsilon: Apoptosis inactive. |
| Protein Name | Caspase-1 Casp-1 Interleukin-1 Beta Convertase Il-1bc Interleukin-1 Beta-Converting Enzyme Ice Il-1 Beta-Converting Enzyme P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 |
| Database Links | Reactome: R-HSA-168638 Reactome: R-HSA-448706 Reactome: R-HSA-5620971 Reactome: R-HSA-6803207 Reactome: R-HSA-844456 Reactome: R-HSA-844615 Reactome: R-HSA-844623 Reactome: R-HSA-9008059 Reactome: R-HSA-9660826 Reactome: R-HSA-9692916 |
| Cellular Localisation | Cytoplasm Cell Membrane |
| Alternative Antibody Names | Anti-Caspase-1 antibody Anti-Casp-1 antibody Anti-Interleukin-1 Beta Convertase antibody Anti-Il-1bc antibody Anti-Interleukin-1 Beta-Converting Enzyme antibody Anti-Ice antibody Anti-Il-1 Beta-Converting Enzyme antibody Anti-P45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 antibody Anti-CASP1 antibody Anti-IL1BC antibody Anti-IL1BCE antibody |
Information sourced from Uniprot.org