• Confocal imaging of paraffin-embedded Mouse brain tissue using Calbindin Rabbit monoclonal antibody (STJ11102032, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat brain tissue using Calbindin Rabbit monoclonal antibody (STJ11102032, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using Calbindin Rabbit monoclonal antibody (STJ11102032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CALB1 antibody (1-100) [S2MR] (STJ11102032)

SKU:
STJ11102032

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Calbindin (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALB1
Gene ID: 793
Uniprot ID: CALB1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Calbindin (P05937).
Immunogen Sequence: MAESHLQSSLITASQFFEIW LHFDADGSGYLEGKELQNLI QELQQARKKAGLELSPEMKT FVDQYGQRDDGKIGIVELAH VLPTEENFLLLFRCQQLKSC
Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Protein Name Calbindin
Calbindin D28
D-28k
Vitamin D-Dependent Calcium-Binding Protein - Avian-Type
Database Links Reactome: R-HSA-977225
Alternative Antibody Names Anti-Calbindin antibody
Anti-Calbindin D28 antibody
Anti-D-28k antibody
Anti-Vitamin D-Dependent Calcium-Binding Protein - Avian-Type antibody
Anti-CALB1 antibody
Anti-CAB27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance